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Comparison of TMT6plex MS3 IT vs OT

ABSTRACT: This dataset is associated with a manuscript where the SPS MS3 method was used for quantifying peptides and a comparison was made between using the ion trap or Orbitrap for the MS3 analysis

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Saccharomyces Cerevisiae (ncbitaxon:4932)

SUBMITTER: Sonja Hess

PROVIDER: MSV000079696 | MassIVE | Tue May 03 09:54:00 BST 2016

SECONDARY ACCESSION(S): PXD004093

REPOSITORIES: MassIVE

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Publications

Improved 6-Plex Tandem Mass Tags Quantification Throughput Using a Linear Ion Trap-High-Energy Collision Induced Dissociation MS(3) Scan.

Liu Jane M JM Sweredoski Michael J MJ Hess Sonja S

Analytical chemistry 20160713 15

The use of tandem mass tags (TMT) as an isobaric labeling strategy is a powerful method for quantitative proteomics, yet its accuracy has traditionally suffered from interference. This interference can be largely overcome by selecting MS(2) fragment precursor ions for high-energy collision induced dissociation (HCD) MS(3) analysis in an Orbitrap scan. While this approach minimizes the interference effect, sensitivity suffers due to the high AGC targets and long acquisition times associated with ...[more]

PMID: 27377715

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