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FLNc Calpain GluC SRM Analysis

ABSTRACT: Human filamin C (FLNc) is a target of the protease calpain at Y2625. To confirm the dependency of calpain cleavage from FLNc phosphorylation at position S2623/S2624 by PKCa, FLNc was overexpressed in HEK293 cells and cells were treated with PMA to stimulated kinase activity, with Gö6976 to inhibit kinase activity or mock-treated with DMSO. Subsequently, recombinant calpain-1 and GluC were used and the resulting peptide TVTSSSSRGSSY was monitored by SRM analyses.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Bettina Warscheid

PROVIDER: MSV000080733 | MassIVE | Wed Mar 29 02:59:00 BST 2017

SECONDARY ACCESSION(S): PXD005097

REPOSITORIES: MassIVE

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