Global analysis of impact of Atg5 mutation on steady-state protein levels

ABSTRACT: This experiment analyzes the global impact of Atg5-/- mutation on steady-state protein levels by a standard SILAC experiment. WT+vector cells were grown in isotopically labeled media for multiple passages in order to fully label the proteome. Unlabeled Atg5-/- cells and labeled WT+vector cells were grown to confluency and protein extracts from quiescent cultures were combined at a 1:1 ratio and analyzed by LC-MS/MS. In this experiment, ratios of labeled to unlabeled spectra report on changes in steady-state expression levels of proteins induced by impairment of autophagy. Table of files Cells Fractionation Raw Data Filenames Wildtype & Atg5-/- Yes SILAC_(1-8).raw (8 fractions)

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Sina Ghaemmaghami  

PROVIDER: MSV000080752 | MassIVE | Wed Mar 29 06:20:00 BST 2017

SECONDARY ACCESSION(S): PXD003558

REPOSITORIES: MassIVE