Proteomics

Dataset Information

0

RPE-1 and RPE-1 trisomic AHA p-c and steady state data


ABSTRACT: We developed a pulse-chase method in where fully SILAC (heavy, medium-heavy and Light) labelled cells were pulsed with Azidohomoalanine (AHA) and then chased for different length of times. In addition, steady state protein levels comparing the RPE- and RPE-1 trisomic cells by standard SILAC labeling was also acquired. These datasets contain data for the human RPE-1 and RPE-1 trisomic cell lines.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo sapiens  

SUBMITTER: Matthias Selbach  

PROVIDER: MSV000080831 | Massive | Fri Mar 31 15:22:00 BST 2017

SECONDARY ACCESSION(S): PXD004915

REPOSITORIES: MassIVE

altmetric image

Publications


Do young and old protein molecules have the same probability to be degraded? We addressed this question using metabolic pulse-chase labeling and quantitative mass spectrometry to obtain degradation profiles for thousands of proteins. We find that >10% of proteins are degraded non-exponentially. Specifically, proteins are less stable in the first few hours of their life and stabilize with age. Degradation profiles are conserved and similar in two cell types. Many non-exponentially degraded (NED)  ...[more]

Similar Datasets

2016-10-06 | PXD004915 | Pride
2016-10-06 | PXD004929 | Pride
2018-09-25 | MSV000082975 | Massive
2016-06-14 | PXD003558 | Pride
2009-10-16 | BIOMD0000000248 | BioModels
2015-01-28 | PXD001555 | Pride
2008-06-13 | E-GEOD-5239 | ArrayExpress
2006-07-07 | GSE5239 | GEO
2017-04-19 | PXD002765 | Pride
2018-01-02 | PXD007621 | Pride