Proteomics

Dataset Information

0

Epigenetic Control of Drought Acclimation in Sorghum (EPICON): Leaf and Root Proteomics

ABSTRACT: Two cultivars of Sorghum biocolor were examined from a study performed at the Kearney Research Station in California; 1) a pre-flowering drought tolerant, post-flowering drought susceptible cultivar RTx430 and 2) a pre-flowering drought susceptible, post-flowering drought-tolerant cultivar BTx642. Samples are from leaves and roots collected at 2, 3, 4, 6, 8, 9, 10, 11, 13, 15, 17 weeks post soil emergence and under a perturbation of either well-watered (control), pre-flowering drought, or post-flowering drought. A liquid-based bottom up proteomics analysis was performed using an iTRAQ labeling multiplexing approach to determine relative abundances. After the peptides were digested with trypsin and iTRAQ labeled, they were fractionated into 12 fractions offline using a C-18 column under high-pH solvent conditions Each fraction was further separated on a low pH C-18 column with direct infusion into a ThermoScientific Q-Exactive mass spectrometer. See the supplementary files for iTRAQ reporter ions details.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Sorghum Bicolor (ncbitaxon:4558)

SUBMITTER: Peggy Lemaux   Christer Jansson  

PROVIDER: MSV000082487 | MassIVE | Thu Jun 21 15:20:00 BST 2018

REPOSITORIES: MassIVE

Json Xml
altmetric image

Publications

Drought stress is a major obstacle to crop productivity, and the severity and frequency of drought are expected to increase in the coming century. Certain root-associated bacteria have been shown to mitigate the negative effects of drought stress on plant growth, and manipulation of the crop microbiome is an emerging strategy for overcoming drought stress in agricultural systems, yet the effect of drought on the development of the root microbiome is poorly understood. Through 16S rRNA amplicon a  ...[more]

Similar Datasets

Proteomics Epigenetic Control of Drought Acclimation in Sorghum (EPICON)
2017-10-23 | MSV000081639 | MassIVE
Proteomics Transient drought during flowering initiation modifies the grain proteome of bread winter wheat
2023-07-28 | PXD040279 | Pride
Unknown RNA-seq analysis of developing rice seeds under combined heat and drought stress
2020-11-04 | GSE153030 | GEO
Transcriptomics Expression data in response to abiotic stresses in tomato at flowering stage
2010-06-23 | E-GEOD-22304 | biostudies-arrayexpress
Transcriptomics RNA-seq analyses of most resistance Indica Rice varieties (Oryza sativa L.) leaves tissue under water-deficit condition
2017-07-27 | E-MTAB-5402 | biostudies-arrayexpress
Proteomics Network analyses and data integration of proteomics and metabolomics from leaves of two contrasting varieties of sugarcane in response to drought
2020-01-02 | PXD014920 | Pride
Proteomics Novel drought adaptation strategies identified by comparing organelle- and cytoplasmic-enriched protein profiles of two Sorghum bicolor genotypes (RTx430 and BTx642) with differing pre-flowering drought tolerance
2020-10-19 | MSV000086332 | MassIVE
Unknown Transcriptome dynamics of rice spikelets under heat stress treatment
2021-01-31 | GSE136746 | GEO
Unknown Effect of drought stress on gene expression of maize leaves
2023-03-31 | GSE223667 | GEO
Transcriptomics Transcriptome profiling of reproductive stage flag leaves of wheat from drought susceptible parent WL711, drought tolerant parent C306 and drought susceptible and drought tolerant RIL bulks in irrigated and drought condition
2012-04-29 | E-GEOD-30436 | biostudies-arrayexpress