Novel drought adaptation strategies identified by comparing organelle- and cytoplasmic-enriched protein profiles of two Sorghum bicolor genotypes (RTx430 and BTx642) with differing pre-flowering drought tolerance

ABSTRACT: Proteomics of organelle and cytoplasmic enriched samples from Sorghum bicolor RTx430 (pre-flowering drought tolerant) and RTx642 (pre-flowering drought sensitive) before and during drought. 3 biological replicates, except BTx642 drought samples, which had 2 biological replicates due to limited sample. Identified multiple protein-level responses in both genotypes, demonstrated that these responses are consistent with the literature, as well as identified multiple RTx430-specific changes that suggest novel drought-tolerance strategies unique to RTx430. Sorghum leaves (both genotypes, +/- drought) were separated into cytosolic and organelle fractions, then digested with trypsin, and analyzed by LC-MS/MS. Data was searched using MaxQuant's Andromeda and a UniProt database. Data was then evaluated for missingness, normalized, and missing values imputed using Bioconductor R package DEP (Zhang et al. 2018).

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Sorghum Bicolor (ncbitaxon:4558)

SUBMITTER: Pubudu P. Handakumbara  

PROVIDER: MSV000086332 | MassIVE | Mon Oct 19 14:10:00 BST 2020

SECONDARY ACCESSION(S): PXD022082

REPOSITORIES: MassIVE