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Metabolic niche adaptation of community- and hospital-associated methicillin-resistant Staphylococcus aureus

ABSTRACT: Acquisition of mass spectrometry (MS) data was performed in the data-independent mode (DIA) mode by shotgun nano-liquid chromatography (LC)-MS/MS on a Q Exactive Plus (Thermo-Fisher Scientific, Waltham, MA, USA) connected to an Ultimate 3000 Nano LC as described before. Prior to the separation of samples by nano-HPLC, an iRT-spike-in-mix (Biognosys AG, Schlieren, Switzerland) was added to the samples. To construct strain-specific ion libraries, samples were measured in data-dependent mode (DDA) as described before. Sequence of the respective isolates was used to search for the database by MaxQuant. The Spectronaut Pulsar software package (v 11.0.15038.12.32941, Biognosys AG 2013) was used to analyse the samples in a strain-dependent manner.

INSTRUMENT(S): Q Exactive plus (Thermo Scientific instrument model)

ORGANISM(S): S. Aureus Usa300

SUBMITTER: Uwe Voelker

PROVIDER: MSV000082578 | MassIVE | Fri Jul 06 09:08:00 BST 2018

REPOSITORIES: MassIVE

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