A viral Argonaute suppressor implements a dual program to block antiviral immunity

ABSTRACT: RAW files accompanying the PPI data in our 2018 Cell Host Microbe manuscript. AP-MS experiments exploring host interactions of the 1A viral suppressor proteins from DCV and CRPV along with mechanistically related experiments (see manuscript for details). Each file corresponds to one gel band from geLC-MS analysis of His-Flag tandem affinity purified bait proteins stably expressed in Drosophila Melanogaster S2 cell line. Each purification was run by SDS-PAGE and the entire lane was cut into 6 gel bands. 3-5 replicate purifications were performed. Five negative control purifications were performed: 2 purifications from untransfected S2 cells, and 3 purifications from tagged eGFP. The data were analyzed by looking at enrichment over the 3 control purifications with highest spectral counts for a given prey using SAINT express. A key mapping experiment to RAW file is provided as an excel file.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Drosophila <fruit Fly, Genus> (ncbitaxon:7215) Cricket Paralysis Virus (ncbitaxon:12136) Drosophila C Virus (ncbitaxon:64279)

SUBMITTER: Raul Andino  

PROVIDER: MSV000082752 | MassIVE | Mon Aug 06 16:38:00 BST 2018

REPOSITORIES: MassIVE