Three-pronged 'Pitchfork' strategy enables an extensive description of human membrane proteome and identification of missing proteins

ABSTRACT: In this study, we combined the transmembrane segment-oriented method hpTC with a solid phase enrichment of glycopeptides (SPEG) and standard 'detergent and trypsin' approach into a three-pronged 'Pitchfork' strategy to maximize membrane proteome coverage. The strategy enabled identification of over 1200 IMPs in human lymphoma cells.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ondrej Vit  

PROVIDER: MSV000083404 | MassIVE | Tue Feb 05 05:02:00 GMT 2019

SECONDARY ACCESSION(S): PXD012599

REPOSITORIES: MassIVE