Proteomics

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20180725 Buthelezi TripleTOF FFPE of human breast tissue


ABSTRACT: Sindisiwe Buthelezi generated these "shotgun" data in July of 2018 on a SCIEX TripleTOF 6600 at the Council for Scientific and Industrial Research in Pretoria, South Africa. The twelve Information-Dependent Acquisition experiments compared one-slide-at-a-time pressure-based paraffin removal (A1-4) with all-at-once paraffin removal (A5-8) with freshly frozen samples (A9-12) using human breast tissue under CSIR Research Ethics Committee reference 213/2017. After conversion to mzML, a total of 474,722 tandem mass spectra were available, of which 39% resulted from doubly-charged precursor ions. Over all twelve experiments, tandem mass spectra were acquired at a rate of 8.40 Hz. MS/MS scans averaged a peak density average of 54 peaks per spectrum.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Stoyan Stoychev  

PROVIDER: MSV000083581 | MassIVE | Fri Mar 15 10:03:00 GMT 2019

SECONDARY ACCESSION(S): PXD013107

REPOSITORIES: MassIVE

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