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MLST8-CCT DSS crosslinking mass spectrometry

ABSTRACT: DSS crosslinking of mLST8-CCT purified from HEK293T cells using a tandem affinity approach. First cell extract was run over HisTrap column then a streptactin column.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Barry Willardson

PROVIDER: MSV000083839 | MassIVE | Wed May 22 13:54:00 BST 2019

SECONDARY ACCESSION(S): PXD013975

REPOSITORIES: MassIVE

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Publications

Structural and functional analysis of the role of the chaperonin CCT in mTOR complex assembly.

Cuéllar Jorge J Ludlam W Grant WG Tensmeyer Nicole C NC Aoba Takuma T Dhavale Madhura M Santiago César C Bueno-Carrasco M Teresa MT Mann Michael J MJ Plimpton Rebecca L RL Makaju Aman A Franklin Sarah S Willardson Barry M BM Valpuesta José M JM

Nature communications 20190628 1

The mechanistic target of rapamycin (mTOR) kinase forms two multi-protein signaling complexes, mTORC1 and mTORC2, which are master regulators of cell growth, metabolism, survival and autophagy. Two of the subunits of these complexes are mLST8 and Raptor, β-propeller proteins that stabilize the mTOR kinase and recruit substrates, respectively. Here we report that the eukaryotic chaperonin CCT plays a key role in mTORC assembly and signaling by folding both mLST8 and Raptor. A high resolution (4.0 ...[more]

PMID: 31253771

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mLST8-CCT DSS crosslinking mass spectrometry

Project description:DSS crosslinking of mLST8-CCT purified from HEK293T cells using a tandem affinity approach. First cell extract was run over HisTrap column then a streptactin column.

2019-05-21 | MSV000083834 | MassIVE

RNA-seq and Mass spectrometry analysis reveals the roles of YTHDF1 in aging

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2021-01-08 | PXD010111 | Pride

Chemical crosslinking of photosystem I antenna proteins

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Combining proximity labeling and crosslinking mass spectrometry for proteomic dissection of nuclear envelope interactome

Project description:Analysis of LAP1 interactome in HEK293T cells using proximity labeling or proximity labeling combined with crosslinking methods.

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Crosslinking mass spectrometry analysis of the Trypanosoma brucei KKT23 complex

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Mapping Hsp104 structure and substrate interactions using crosslinking mass spectrometry

Project description:Hsp104_wild_type; LUM2_S11709; ADH_DMTMM_ADH. Hsp104_wild_type; LUM2_S11710; ADH_DMTMM; Hsp104_wild_type; LUM1_S10782; DSS Hsp104_mutant; LUM2_S11711; ADH_DMTMM_ADH Hsp104_mutant; LUM2_S11712; ADH_DMTMM Hsp104_mutant; LUM1_S10783; DSS Hsp104_PCSK9; LUM2_S11713; ADH_DMTMM_ADH Hsp104_PCSK9; LUM2_S11714; ADH_DMTMM Hsp104_PCSK9; LUM1_S11618; DSS

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2026-01-21 | PXD061766 | Pride

26S proteasome crosslinking mass spectrometry data for mi-filter

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Crosslinking mass spectrometry of the α/β subunit interface in urinary and recombinant human chorionic gonadotropin (hCG)

Project description:Human chorionic gonadotropin (hCG) is a heterodimeric glycoprotein hormone whose urinary (u-hCG) and recombinant (r-hCG) forms differ in glycosylation and stability. To test whether this reflects a structural reorganization of the α/β subunit interface, the two forms were compared by chemical crosslinking mass spectrometry (XL-MS). Crosslinking was performed on the intact, natively glycosylated heterodimers using two complementary chemistries: the lysine-reactive reagent DSS and the zero-length coupling reagent DMTMM (targeting Lys-Asp/Glu pairs, each in biological triplicate for both forms. Identified inter-subunit crosslinks were mapped onto the hCG structure to evaluate whether the assembled core interface is conserved between u-hCG and r-hCG. The dataset comprises the raw LC-MS/MS files and the corresponding crosslink identifications for all conditions and replicates, and accompanies a broader native-MS, ion-mobility and collision-induced-unfolding study of the two hCG forms.

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