A novel translocon for biogenesis of multi-pass membrane proteins

ABSTRACT: A novel 370 kDa translocon comprising Sec61 and five accessory factors acting co-translationally with ribosomes to fold and insert multi-pass membrane proteins was identified, purified and analyzed structurally by single particle cryo-EM and cross-linking mass spectrometry. The purified ribosome-translocon complex was cross-linked with DSS, trypsin digested, and size-exclusion enriched (SEC) cross-link containing fractions were analyzed by sequential HCD/ETD of the same precursor ion. The four LC-MS runs corresponding to SEC fractions eluting between 0.9-1.4 ml are deposited here.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Robert J. Keenan  

PROVIDER: MSV000084065 | MassIVE | Mon Jul 08 11:56:00 BST 2019

REPOSITORIES: MassIVE