Human pluripotent stem cell differentiation into hepatocyte-like cells in microfluidics: secretome and intracellular proteome.

ABSTRACT: Human pluripotent stem cells (both embryonic and induced pluripotent by reprogramming) were differentiated into hepatic-like cells inside microfluidic culture chambers and in conventional culture systems. Conditioned media and cell lysates were harvested and analyzed. Before starting the differentiation protocol, cells were labeled with the technique "stable isotope labeling with amino acids in cell culture" (SILAC). Then, heavy cells were used in microfluidics and light cells were used in conventional culture system for a relative quantitative comparison.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Nicola Elvassore  

PROVIDER: MSV000084128 | MassIVE | Wed Jul 24 00:45:00 BST 2019

SECONDARY ACCESSION(S): PXD014729

REPOSITORIES: MassIVE