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Human pluripotent stem cell differentiation into hepatocyte-like cells in microfluidics: secretome and intracellular proteome.

ABSTRACT: Human pluripotent stem cells (both embryonic and induced pluripotent by reprogramming) were differentiated into hepatic-like cells inside microfluidic culture chambers and in conventional culture systems. Conditioned media and cell lysates were harvested and analyzed. Before starting the differentiation protocol, cells were labeled with the technique "stable isotope labeling with amino acids in cell culture" (SILAC). Then, heavy cells were used in microfluidics and light cells were used in conventional culture system for a relative quantitative comparison.

INSTRUMENT(S): Orbitrap Fusion ETD

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Nicola Elvassore

PROVIDER: MSV000084128 | MassIVE | Wed Jul 24 00:45:00 BST 2019

SECONDARY ACCESSION(S): PXD014729

REPOSITORIES: MassIVE

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Publications

The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs.

Michielin Federica F Giobbe Giovanni G GG Luni Camilla C Hu Qianjiang Q Maroni Ida I Orford Michael R MR Manfredi Anna A Di Filippo Lucio L David Anna L AL Cacchiarelli Davide D De Coppi Paolo P Eaton Simon S Elvassore Nicola N

Cell reports 20201201 9

The specification of the hepatic identity during human liver development is strictly controlled by extrinsic signals, yet it is still not clear how cells respond to these exogenous signals by activating secretory cascades, which are extremely relevant, especially in 3D self-organizing systems. Here, we investigate how the proteins secreted by human pluripotent stem cells (hPSCs) in response to developmental exogenous signals affect the progression from endoderm to the hepatic lineage, including ...[more]

PMID: 33264615

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