Proteomic profile of human Schwann cells

ABSTRACT: Schwann cells (SC) are essential to the growth, maintenance and regeneration of peripheral nerves, but the proteome of normal human SC is poorly defined. Here, we performed a proteomic analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS) to identify the protein expression profile of primary human SC. A total of 19,557 peptides corresponding to 1,553 proteins were identified, with 1% false discovery rate (FDR). Ingenuity Pathway Analysis (IPA), Gene Ontology (GO) and Database for Annotation, Visualization and Integrated Discovery (DAVID) were used to assign protein localization and function, and to define enriched pathways. EIF2, mTOR and integrin signalling were among the most enriched pathways and the most enriched biological function was cell-cell adhesion, which is in agreement with the supportive role of SC in peripheral nerves. This proteome analysis of normal human SC may help in the molecular exploration of neurological and malignant diseases where SC are involved.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Hubert Hondermarck   Aysha Ferdoushi   Muhammad Fairuz Jamaluddin   Xiang Li  

PROVIDER: MSV000084303 | MassIVE |

REPOSITORIES: MassIVE