ABSTRACT: Extraction of roots from bacterized seeds with Bacillus subtilis subsp subtilis NCBI 3610 and derivated mutants in extracellular matrix components.
INSTRUMENT(S): Q Exactive
ORGANISM(S): Bacillus Subtilis Subsp. Subtilis Str. Ncib 3610 Cucumis Melo
Project description:Extraction of roots from bacterized seeds with Bacillus subtilis subsp subtilis NCBI 3610 and derivated mutants in extracellular matrix components.
Project description:To explore the effects of different stress conditions on Bacillus subtilis str.168, a selection of conditions were applied to the organism and RNA-seq data gathered. A matrix of gene counts was produced as a basis for further analysis into the transcription profiles of Bacillus subtilis str.168.
Project description:Plant growth-promoting rhizobacteria (PGPR) are soil beneficial microorganisms that colonize plant roots for nutritional purposes and accordingly benefit plants by increasing plant growth or reducing disease. But it still remains unclear which mechanisms or pathways are involved in the interactions between PGPR and plants. To understand the complex plant-PGPR interactions, the changes in the transcriptome of typical PGPR standard Bacillus subtilis in responding to rice seedlings were analyzed. We compared and anylyzed the transcriptome changes of the bacteria Bacillus subtilis OKB105 in response to rice seedings for 2 h. Total RNA was extracted and Random priming cDNA synthesis, cDNA fragmentation and terminal labeling with biotinylated GeneChip DNA labeling reagent, and hybridization to the Affymetrix GeneChip Bacillus subtilis Genome Array.
Project description:To compare the altered transcript levels in the floT, floA single and floTfloA double mutants of Bacillus subtilis, cells were grown in MSgg medium until the late exponential phase and their transcriptome was compared to the wild type, NCIB3610 strain. Bacillus subtilis NCIB3610 wild type and its floT, floA, and floTfloA mutants strain were grown in MSgg medium and cells were harvested at late exponential (OD600 ~1.0-1,2) phase. 3 biological replicates were used, resulting 3 slides for each mutant strain. Dye swaps are included in both experiments.
Project description:To compare the altered transcript levels in the floT, floA single and floTfloA double mutants of Bacillus subtilis, cells were grown in MSgg medium until the late exponential phase and their transcriptome was compared to the wild type, NCIB3610 strain.