Evaluation of the effects of sample size on affinity purification-mass spectrometry

ABSTRACT: An affinity purification experiment was carried out to evaluate the effects of sample size on the performance of AP-MS experiments. The Arp2/3 complex was affinity purified from Saccharomyces cerevisiae cells containing GFP-tagged Arc35, a core subunit of the complex, in-gel digested by trypsin, and label-free differential mass spectrometry was used for the unbiased analysis of detected molecular features. By comparing the performance of the full dataset (n=6 affinity purifications per condition) and resampled data subsets of different sample sizes (n=3 to 5 affinity purifications per condition), we showed that larger sample size not only increased the number of significant known interactors but also bettered their significance ranking.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Saccharomyces Cerevisiae By4741 (ncbitaxon:1247190)

SUBMITTER: Nathan A Yates  

PROVIDER: MSV000085564 | MassIVE | Mon Jun 08 13:52:00 BST 2020

SECONDARY ACCESSION(S): PXD019656

REPOSITORIES: MassIVE