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Collision energies on QTof and Orbitrap instruments: How to make proteomics measurements comparable?

ABSTRACT: Comparison of LeuEnk, enolase, and HeLa tryptic peptides MS/MS spectra obtained on a Bruker QTof CID and a Thermo Q-Exactive Focus Orbitrap HCD instrument as a function of collision energy using the similarity index.

INSTRUMENT(S): maXis, Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Agnes Revesz

PROVIDER: MSV000086434 | MassIVE | Wed Nov 11 02:48:00 GMT 2020

REPOSITORIES: MassIVE

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Collision energies on QTof and Orbitrap instruments: How to make proteomics measurements comparable?

Szabó Dániel D Schlosser Gitta G Vékey Károly K Drahos László L Révész Ágnes Á

Journal of mass spectrometry : JMS 20201205 1

Quadrupole time-of-flight (QTof) collision-induced dissociation (CID) and Orbitrap higher-energy collisional dissociation (HCD) are the most commonly used fragmentation techniques in mass spectrometry-based proteomics workflows. The information content of the MS/MS spectra is first and foremost determined by the applied collision energy. How can we set up the two instrument types to achieve maximum transferability? To answer this question, we compared MS/MS spectra obtained on a Bruker QTof CID ...[more]

PMID: 33277714

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