Proteomics

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The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

ABSTRACT: Proteomic data from pulldown performed with the recombinant Campylobacter CiaD-GST fusion was expressed in E. coli BL21(DE3) on a lysate of human cultured INT 407 cells. One non-specific binding control was performed on INT 407 cell lysates and two-pulldowns were realized with the recombinant protein. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Geremy Clair  

PROVIDER: MSV000086662 | MassIVE | Tue Jan 05 10:43:00 GMT 2021

SECONDARY ACCESSION(S): PXD023421

REPOSITORIES: MassIVE

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The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry.

Negretti Nicholas M NM   Gourley Christopher R CR   Talukdar Prabhat K PK   Clair Geremy G   Klappenbach Courtney M CM   Lauritsen Cody J CJ   Adkins Joshua N JN   Konkel Michael E ME  

Nature communications 20210226 1

Campylobacter jejuni is a foodborne pathogen that binds to and invades the epithelial cells lining the human intestinal tract. Maximal invasion of host cells by C. jejuni requires cell binding as well as delivery of the Cia proteins (Campylobacter invasion antigens) to the host cell cytosol via the flagellum. Here, we show that CiaD binds to the host cell protein IQGAP1 (a Ras GTPase-activating-like protein), thus displacing RacGAP1 from the IQGAP1 complex. This, in turn, leads to the unconstrai  ...[more]

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