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Proteomics on the ovaries of failed and healthy honey bee queens

ABSTRACT: We sampled honey bee queens from beekeepers throughout British Columbia which were rated as either 'failed' or 'healthy' by beekeepers. We performed shot-gun proteomics on the ovaries. Proteins were reduced, alkylated, and digested with Lys-C for 4 h in urea digestion buffer. The sample was then diluted 4x with ammonium bicarbonated and digested overnight with trypsin. Peptides were analyzed by an EASY-nLC 1000 liquid chromatography system coupled to a Bruker Impact II mass spectrometer. Data were searched with MaxQuant and the zipped txt folder output is provided.

INSTRUMENT(S): impact II

ORGANISM(S): Apis Mellifera (ncbitaxon:7460)

SUBMITTER: Leonard Foster

PROVIDER: MSV000087150 | MassIVE | Fri Apr 02 09:37:00 BST 2021

REPOSITORIES: MassIVE

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