Identification of human transglutaminase 4 substrates in AD-293 protein extract by biotin-pentylamine incorporation

ABSTRACT: We have identified cellular substrates of human transglutaminase 4 (TG4) by LC-MS/MS-based method from transglutaminase negative AD-293 cell line. Recombinant human TG4 dependent biotin pentylamine (BPA) modified peptides/proteins were detected from cytoplasmic (Cp) and nuclear (N) protein fractions of AD-293 cells (performed with 3 parallels). Control shows the neutravidin agarose interactome of AD-293 protein extract.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Ilma Rita Korponay-Szabo and Robert Kiraly  

PROVIDER: MSV000088030 | MassIVE | Fri Aug 27 15:25:00 BST 2021

SECONDARY ACCESSION(S): PXD028174

REPOSITORIES: MassIVE