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Multi-scan cycle time centric optimization allows rapid intact protein and top down characterization

ABSTRACT: The ionization conditions necessary for intact protein characterization in Orbitrap mass analyzers can be highly variable. To more rapidly characterize intact proteins we have developed a method where multiple sets of conditions can be used within a single experiment. These 3 scan events contain generic parameters for: 1) Proteins that do not require additional in-source collision energy for efficient ionization, 2) Proteins that require some additional energy, 3) Proteins that require substantial in source energy. We test this method with a commercial intact protein standard containing proteins between 10kDa and 90kDa with and without the addition of the NIST monoclonal antibody standard.

INSTRUMENT(S): Exactive, Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Benjamin C Orsburn

PROVIDER: MSV000088590 | MassIVE | Mon Dec 20 02:22:00 GMT 2021

REPOSITORIES: MassIVE

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