Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 1000

Alison Hughes Thesis - Chapter 3: Extraction Solvent

ABSTRACT: Chlorella sorokiniana cultures extracted using ethyl acetate, methanol, butanol, and butanol:dichloromethane (1:1)

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Chlorella Sorokiniana (ncbitaxon:3076)

SUBMITTER: Katherine Duncan

PROVIDER: MSV000088662 | MassIVE | Sat Jan 08 05:34:00 GMT 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS Alison Hughes Thesis - Chapter 3: Extraction Solvent

Project description:Chlorella sorokiniana cultures extracted using ethyl acetate, methanol, butanol, and butanol:dichloromethane (1:1)

2022-01-08 | MSV000088662 | GNPS

Untargeted LC-MS/MS analysis of microbes associated with Odontotaenius disjunctus (bessbug beetle) frass

Project description:Microbes were isolated from frass material collected within laboratory galleries of Odontotaenius disjunctus (bessbug beetles) in the USA, and cultured on ISP2-agar for seven days at 30C. Cultures were extracted with ethyl acetate or methanol.

2022-07-31 | MSV000090030 | MassIVE

PerezLorente_and_MolinaSantiago-microbeMASST-Collaboration

Project description:Monocultures of bacteria or fungi. Samples were extracted using methanol 80% or ethyl-acetate.

2022-07-14 | MSV000089887 | MassIVE

Methylorubrum extorquens supernatant extract use case

Project description:Three independent cultures of Methylorubrum extorquens PA1 delta cel were grown on ammonium mineral salts with either methanol or succinate provided as the sole carbon and energy source. The supernatant was subsequently extracted with acidified ethyl acetate and analyzed by LC-MS.

2021-02-10 | MSV000086834 | MassIVE

Metaproteomic analysis of dichloromethane-fermenting enrichment culture DFE

Project description:Enrichment culture DFE is capable of fermenting the toxic pollutant dichloromethane (DCM; CH2Cl2) into environmentally benign acetate. The dominant organism in the enrichment culture is a novel bacterium in the Peptococcaceae family, “Candidatus Formimonas warabiya” strain DCMF. This metaproteomic study analysed culture DFE during growth with four substrates – DCM, glycine betaine, choline, and methanol – all of which are primarily, if not solely, metabolised by strain DCMF.

2023-03-11 | PXD037334 | Pride

20230327_ Alicia_David_Botrytis_dipeptide

Project description:Botrytis treated with cyclodipeptide. Cell and supernatant fraction extracted with methanol and ethyl-acetate, respectively.

2023-03-27 | MSV000091558 | MassIVE

Differential analysis of Methylobacterium extorquens DM4 in methanol versus dichloromethane condition

Project description:Differential analysis of Methylobacterium extorquens DM4 in methanol versus dichloromethane condition using shotgun label free MS1 quantification approach

2018-04-25 | PXD006838 | Pride

In vitro_PKS14-overexpressing strain

Project description:PKS14-overexpressing strain was inoculated into PDB for 7 days. The fungal cells were extracted with methanol, and the culture broth was extracted with ethyl acetate.

2023-01-02 | MSV000090990 | MassIVE

Arabidopsis thaliana peptide SP2 and ethyl acetate extraction clean up.

Project description:The success of bottom-up proteomic analysis frequently depends on the efficient removal of contaminants from protein or peptide samples before LC-MS/MS. For a peptide clean-up workflow, the single-pot solid-phase-enhanced peptide sample preparation on carboxylate-modified paramagnetic beads (termed SP2) was evaluated for sodium dodecyl sulfate or polyethylene glycol removal from Arabidopsis thaliana tryptic peptides. The robust and efficient 40-min SP2 protocol, tested for a 10 ng, 250ng and 10µg peptide sample, was proposed and benchmarked thoroughly against the ethyl acetate extraction protocol. The SP2 protocol on carboxylated magnetic beads proved to be the robust approach even for simultaneous removal of massive sodium dodecyl sulfate (SDS) and polyethylene glycol (PEG) contaminations from AT peptide samples in respect of the LC-MS/MS data outperforming ethyl acetate extraction.

2024-01-03 | PXD045031 | Pride

In vitro_PKS14-knockout strain

Project description:The PKS14-knockout strain was inoculated into PDB for 7 days. The fungal cells were extracted with methanol, and the culture broth was extracted with ethyl acetate.

2023-01-02 | MSV000090991 | MassIVE

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data