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MAG alters the secretion of proteins involved in CNS plasticity

ABSTRACT: Myelin-associated glycoprotein (MAG) is a potent inhibitor of neurite growth in the central nervous system. Label free LC-MS2 quantitation was performed on the secretome from three sets of rat neuron cultures in two states: control, and exposed to MAG. Differential analysis of conditioned media revealed that MAG exposure up-regulates LTBP3, LTBP4, S100A6, septin-7 and neurofascin 186, while down-regulating frataxin, MAP6, syntenin-1 and GAP-43. When combined with other results from this study, these findings confirm that MAG induces secretion of transforming growth factor beta, which increases the expression of chondroitin sulfate proteoglycans after spinal cord injury. They also demonstrate that MAG has a generalized effect on the secretion of proteins that affect CNS plasticity - increasing the secretion of growth-inhibiting proteins, while suppressing the secretion of proteins that promote axonal growth. These are new insights into the mechanisms underlying MAG's ability to inhibit axonal regeneration.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116)

SUBMITTER: Sari Hannila

PROVIDER: MSV000089160 | MassIVE | Wed Mar 30 08:18:00 BST 2022

REPOSITORIES: MassIVE

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