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Pulse SILAC for MEFs to determine the new-synthesized protien after FBS treatment

ABSTRACT: We are trying to determine the new synthesized proteinns after FBS treatment. We used R6/K4 and R10/K8 to lable the new synthesized proteins. Wild-type mouse embryonic fibroblasts (MEFs) were cultured in regular arginine and lysine (R0/K0) medium and starved in serum-free medium for 4 hours before treatment. R6/K4 or R10/K8 medium with or without FBS was used to culture MEFs for 6 hours. Total proteins were harvested and mixed at equal amount for LC/MS.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Zhao Wang

PROVIDER: MSV000089184 | MassIVE | Thu Mar 31 11:49:00 BST 2022

REPOSITORIES: MassIVE

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