Dataset Information

Mechanical loading but not insertion into an enthesis is required for initiaion of myotendinous junction development

ABSTRACT: Data files included in this submission are from 4 separate experiments to show that myotendinous junction formation and muscle maturation is dependent on muscle contraction. For Tbx3 E14.5 and E18.5 CS and IN samples: forelimbs (n=3 each genotype and age) were fractionated into C, N, M, CS, and IN fractions and proteins in CS and IN were identified by shotgun proteomics. For Tbx3 P21 and mdg E18.5 samples: samples were homogenized in high-molar urea and proteins were identified by shotgun proteomics.

INSTRUMENT(S): Q Exactive HF-X, Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Sarah Calve

PROVIDER: MSV000089551 | MassIVE | Thu May 26 10:00:00 BST 2022

REPOSITORIES: MassIVE

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Publications

Mechanical loading is required for initiation of extracellular matrix deposition at the developing murine myotendinous junction.

Lipp Sarah N SN Jacobson Kathryn R KR Colling Haley A HA Tuttle Tyler G TG Miles Dalton T DT McCreery Kaitlin P KP Calve Sarah S

Matrix biology : journal of the International Society for Matrix Biology 20230126

The myotendinous junction (MTJ) contributes to the generation of motion by connecting muscle to tendon. At the adult MTJ, a specialized extracellular matrix (ECM) is thought to contribute to the mechanical integrity of the muscle-tendon interface, but the factors that influence MTJ formation during mammalian development are unclear. Here, we combined 3D imaging and proteomics with murine models in which muscle contractility and patterning are disrupted to resolve morphological and compositional ...[more]

PMID: 36709857

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Project description:Smooth muscle cells (SMCs) are important in a number of physiological systems and organs, including the cardiovascular system. The hallmark property of differentiated SMCs is the ability to contract, but contractile SMCs themselves show a range of phenotypes allowing prolonged tonic contraction in vascular smooth muscle or rapid phasic contraction in tissues such as bladder. Another distinctive characteristic, in contrast with terminally differentiated striated muscle cells, is that SMCs exhibit phenotypic plasticity. Vascular SMCs are able to modulate their phenotype along a continuum between a contractile phenotype, characteristic of healthy blood vessels, and a more proliferative âsyntheticâ phenotype, so-named for the enhanced synthesis and secretion of extracellular matrix components. Synthetic phenotype cells are found in a number of pathological situations such as atherosclerosis and arterial injury. We used mouse exon-junction (MJAY) arrays to gain insights into both the global contribution of alternative splicing events in re-shaping the transcriptome of dedifferentiating mouse aorta and bladder SMCs, and into the underlying regulatory mechanisms of the alternative splicing program. Affymetrix splice junction arrays (MJAY) were used to profile changes in both alternative splicing and transcript levels during the phenotypic modulation of smooth muscle cells when placed in culture. RNA extracted from intact aorta and bladder smooth muscle tissue was used for differentiated samples. For dedifferentiated, proliferative samples smooth muscle cells were enzymatically dispersed and grown in tissue culture for a week. Triplicate RNA samples were prepared from smooth muscle tissue of mouse aorta and bladder (differentiated) and from smooth muscle cells from each tissue cultured for 7 days (proliferative). The samples allowed comparison of alternative splicing (and other transcriptome) changes between differentiated and proliferative smooth muscle cell samples from two distinct types of smooth muscle cell, as well as allowing direct comparison of aorta (tonic smooth muscle) and bladder (phasic smooth muscle).

Dataset Information

Mechanical loading but not insertion into an enthesis is required for initiaion of myotendinous junction development

Publications

Mechanical loading is required for initiation of extracellular matrix deposition at the developing murine myotendinous junction.

Similar Datasets

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