Temporal Proteomic Analyses of Human Lung Cells Infected with Coronavirus OC43

ABSTRACT: The coronaviruses consist of many animal and human pathogens, with some of the human coronavirus, such as strain OC43, normally causing only mild cold-like symptoms. Viruses usurp host cellular processes to successfully replicate. We used tandem mass tag mass spectrometry-based proteomic analyses of human lung MRC5 cells infected with OC43 for various periods of time to delineate virus-induced host cell alterations. Numerous proteins involved in lipid metabolism, molecular transport, small molecule biochemistry, cell death and survival, humoral immune response, and inflammatory response were dysregulated. Comparison of our data with previous studies that examined a continuum of differentially pathogenic influenza viruses, and with SARS-CoV-2 data from other studies, indicated that proteins involved in the cell cycle, cytokine signaling, DNA replication and anti-inflammatory responses were generally similarly affected by virtually all IAV and CoV tested, whereas proteins involved in necrosis, protein metabolism, ECM regulation and signal transduction were generally differentially affected by CoV and IAV. In addition, the more pathogenic CoV and IAV activated Rb-dependent repression of E2F-mediated transcription, whereas less pathogenic influenza and coronaviruses either inhibited or had no effect on this pathway.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Kevin Coombs  

PROVIDER: MSV000089582 | MassIVE | Wed Jun 01 13:02:00 BST 2022

REPOSITORIES: MassIVE