Rapid proximity biotinylation of the Plasmodium falciparum exported protein, SBP1

ABSTRACT: The deadly human malaria-causing parasite, Plasmodium falciparum relies on its capacity to completely remodel its host red blood cell (RBC) through the export of several hundred parasite proteins across several membranes to the RBC.We fused the exported membrane protein, skeleton binding protein 1 (SBP1), with the rapid, efficient, promiscuous biotin ligase known as TurboID (SBP1TbID). Using time-resolved, proximity biotinylation and label-free quantitative proteomics, we identified early (pre-export) interactors and late (post-export) interactors of SBP1TbID.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Plasmodium Falciparum Nf54 (ncbitaxon:5843)

SUBMITTER: Vasant Muralidharan  

PROVIDER: MSV000089743 | MassIVE | Mon Jun 27 17:05:00 BST 2022

SECONDARY ACCESSION(S): PXD034946

REPOSITORIES: MassIVE