Dataset Information

20S proteasomes exported by the malaria parasite promote its growth

ABSTRACT: Mature Red Blood Cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. Pf, while growing inside its natural host, the human RBC, secretes multipurpose extracellular vesicles (EVs), yet their influence on this essential host cell remains unknown. Here we demonstrate that Pf parasites, cultured in fresh human donor blood, export within such EVs assembled and functional 20S proteasome complexes (EV-20S). The EV-20S proteasomes modulate the mechanical properties of naïve human RBCs by remodeling their cytoskeletal network. Furthermore, we identify four novel degradation targets of the exported 20S proteasome, the phosphorylated cytoskeletal proteins β-adducin, ankyrin-1, dematin and Epb4.1. Overall, our findings reveal a previously unknown 20S proteasome export mechanism employed by the human malaria parasite, which primes RBCs for parasite invasion by altering membrane stiffness, to facilitate malaria parasite growth.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Plasmodium Falciparum (isolate 3d7)

TISSUE(S): Erythrocyte, Blood

DISEASE(S): Plasmodium Falciparum Malaria

SUBMITTER: Eugene Kapp

LAB HEAD: Neta Regev-Rudzki

PROVIDER: PXD023353 | Pride | 2021-02-04

REPOSITORIES: Pride

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Publications

20S proteasomes secreted by the malaria parasite promote its growth.

Dekel Elya E Yaffe Dana D Rosenhek-Goldian Irit I Ben-Nissan Gili G Ofir-Birin Yifat Y Morandi Mattia I MI Ziv Tamar T Sisquella Xavier X Pimentel Matthew A MA Nebl Thomas T Kapp Eugene E Ohana Daniel Yael Y Karam Paula Abou PA Alfandari Daniel D Rotkopf Ron R Malihi Shimrit S Temin Tal Block TB Mullick Debakshi D Revach Or-Yam OY Rudik Ariel A Gov Nir S NS Azuri Ido I Porat Ziv Z Bergamaschi Giulia G Sorkin Raya R Wuite Gijs J L GJL Avinoam Ori O Carvalho Teresa G TG Cohen Sidney R SR Sharon Michal M Regev-Rudzki Neta N

Nature communications 20210219 1

Mature red blood cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. Pf, while growing inside its natural host, the human RBC, secretes multipurpose extracellular vesicles (EVs), yet their influence on this essential host cell remains unknown. Here we demonstrate that Pf parasites, cultured in fresh human donor blood, secret ...[more]

PMID: 33608523

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Dataset Information

20S proteasomes exported by the malaria parasite promote its growth

Publications

20S proteasomes secreted by the malaria parasite promote its growth.

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