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CMyBPC_dephosphorylation_PP1_PP2A

ABSTRACT: Bisphosphorylated cMyBP-C following dephosphorylation of pS279,pS288,pS308,pS313-cMyBPC by PP1 or PP2A. Samples analysed by the BIOSCIENCE TECHNOLOGY FACILITY, METABOLOMICS AND PROTEOMICS LABORATORY (BTF-MAP lab) of the University of York.

INSTRUMENT(S): Enriched phosphopeptides were loaded onto a 50 cm EN C18 PepMap column with elution over a 1 h acquisition driven by a Waters mClass nanoUPLC. Eluted peptides were measured using a Thermo Orbitrap Fusion Tribrid mass spectrometer, with MS spectra acquired in the Orbitrap and MS2 spectra acquired in parallel, following HCD fragmentation, in the linear ion trap.

ORGANISM(S): Rattus Norvegicus (ncbitaxon:10116)

SUBMITTER: Daniel Koch

PROVIDER: MSV000091995 | MassIVE | Fri May 19 15:23:00 BST 2023

REPOSITORIES: MassIVE

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