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KLF6PODTA-Conditioned media and urine proteomics

ABSTRACT: Tandem mass spectrometry was performed on the supernatant from mouse primary podocytes isolated from KLF6PODTA and NPHS2-rtTA mice (with and without doxycycline) using a label-free proteomic approach. After the cells reached confluency, cells were washed five times with PBS and medium was replaced with phenol-red, insulin-transferrin-selenium (ITS), and serum-free RPMI. After 48 additional hours, supernatant (conditioned media) was harvested and centrifuged for 5 min at 500 g and then for 10 min at 1500 g. Samples were reduced (dithiothreitol), alkylated (iodoacetamide), digested with trypsin, and cleaned using hydrophobic-lypophilic-balance (HLB) pack C-18.

INSTRUMENT(S): Q Exactive HF Hybrid Quadrupole-Orbitrap

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Sandeep Mallipattu

PROVIDER: MSV000092123 | MassIVE | Wed Jun 07 08:50:00 BST 2023

REPOSITORIES: MassIVE

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