Project description:This study focuses on the untargeted comparative metabolomics analysis of the endo-metabolome of both WT and acot-15 mutant C. elegans worms. Hydroxylamine was used to treat certain samples to capture the reactive electrophilic metabolites.
Project description:This study focuses on the untargeted comparative metabolomics analysis of the endo-metabolome of both WT and acot-15 mutant C. elegans worms. Hydroxylamine was used to treat certain samples to capture the reactive electrophilic metabolites.
Project description:Investigation of whole genome gene expression level changes in C. elegans DAF-15 neuronal AID worms under 5-Ph-IAA treatment. This project contains 8 samples (4 treated by etoh and 4 treated by 5-Ph-IAA) of RNA-seq data.
Project description:MDT-15/MED15 is a subunit of the Mediator complex and is known to regulate specific gene programs. We had previously profiled gene expression in C. elegans following mdt-15 depletion by RNAi. To complement this, we also performed gene expression profiling of the mdt-15(tm2182) hypomorhic mutant to identify MDT-15 regulated genes and developmental and/or physiological gene programs.
Project description:CDK-8 is the catalytic subunit of the Mediator complex and is known to regulate specific gene programs in yeast and in cultured mammalian cells; because no study has yet identified CDK-8 regulated genes in an in vivo animal model, we performed genome wide expression profiling on cdk-8 null mutant C. elegans worms to identify CDK-8 regulated genes and developmental and/or physiological gene programs.
Project description:Most aging hypotheses revolve around the accumulation of some sort of damage resulting in gradual physiological decline and ultimately death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend lifespan. However, lowering translational efficiency extends rather than shortens lifespan in C. elegans. We studied turnover of individual proteins in the conserved Insulin/Insulin-like Growth Factor (IGF-1) receptor mutant daf-2 by combining Stable Isotope Labeling by Nitrogen-15 in Caenorhabditis elegans and LC-MS/MS. Intriguingly, the majority of proteins displayed prolonged half-lives in daf-2, while others remained unchanged, signifying that longevity is not supported by high protein turnover. This slow-down of protein turnover was most prominent for components of the translation machinery and mitochondria. In contrast, the high turnover of lysosomal hydrolases and very low turnover of cytoskeletal proteins remained largely unchanged in daf-2. The slow-down of protein dynamics and decreased abundance of the translational machinery may point at the importance of anabolic attenuation in lifespan extension as suggested by the hyperfunction theory.
Project description:Transcriptional profiling of C. elegans young adult worms cultured on non-pathogenic Bacillus strain 67 versus versus age-matched worms cultured on the control lab food E. coli OP50. The goal was to identify genes regulated in response to differences in diet, which might confer immunity to later exposures to pathogenic Bacillus thuringiensis DB27. One-condition experiment. C. elegans young adults, cultured on : Bacillus strain 67 versus E. coli OP50. 4 biological replicates, including 2 dye-swaps.
Project description:Complex lipid metabolism plays a crucial role in regulating aging. We recently discovered that the phospholipid bis(monoacylglycero)phosphate (BMP) increases in aged human muscles and many mouse tissues. The phospholipase PLA2G15 is reportedly involved in BMP synthesis, however, its specific role in aging remains unknown. To elucidate the role of PLA2G15 in aging, we used C. elegans as a model. When silencing plag-15, the predicted worm orthologue of PLA2G15, we observed improved healthspan and lifespan extension. Semi-targeted lipidomics highlighted that instead of changes related to BMP, plag-15 RNAi led to lower levels of lysophosphatidic acid, lysophosphatidylcholine and lysophosphatidylethanolamine. Transcriptome-guided epistasis experiments identified that the lifespan extension of plag-15 RNAi worms is regulated by transcription factors hlh-30 and elt-3, and lysosomal vitamin B12 transporter pmp-5 (human TFEB, GATA, and ABCD4 respectively). Overall, we conclude that targeting phospholipid remodeling through plag-15 could be a promising strategy to promote healthy aging.