Proteomics

Dataset Information

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Wt vs daf-2 C. elegans protein turnover


ABSTRACT: Most aging hypotheses revolve around the accumulation of some sort of damage resulting in gradual physiological decline and ultimately death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend lifespan. However, lowering translational efficiency extends rather than shortens lifespan in C. elegans. We studied turnover of individual proteins in the conserved Insulin/Insulin-like Growth Factor (IGF-1) receptor mutant daf-2 by combining Stable Isotope Labeling by Nitrogen-15 in Caenorhabditis elegans and LC-MS/MS. Intriguingly, the majority of proteins displayed prolonged half-lives in daf-2, while others remained unchanged, signifying that longevity is not supported by high protein turnover. This slow-down of protein turnover was most prominent for components of the translation machinery and mitochondria. In contrast, the high turnover of lysosomal hydrolases and very low turnover of cytoskeletal proteins remained largely unchanged in daf-2. The slow-down of protein dynamics and decreased abundance of the translational machinery may point at the importance of anabolic attenuation in lifespan extension as suggested by the hyperfunction theory.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Caenorhabditis elegans  

TISSUE(S): Whole Body

DISEASE(S): Not Available

SUBMITTER: Vlad Petyuk  

LAB HEAD: Vlad Petyuk

PROVIDER: PXD002317 | Pride | 2016-09-27

REPOSITORIES: pride

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Publications

FOXO/DAF-16 Activation Slows Down Turnover of the Majority of Proteins in C. elegans.

Dhondt Ineke I   Petyuk Vladislav A VA   Cai Huaihan H   Vandemeulebroucke Lieselot L   Vierstraete Andy A   Smith Richard D RD   Depuydt Geert G   Braeckman Bart P BP  

Cell reports 20160901 11


Most aging hypotheses assume the accumulation of damage, resulting in gradual physiological decline and, ultimately, death. Avoiding protein damage accumulation by enhanced turnover should slow down the aging process and extend the lifespan. However, lowering translational efficiency extends rather than shortens the lifespan in C. elegans. We studied turnover of individual proteins in the long-lived daf-2 mutant by combining SILeNCe (stable isotope labeling by nitrogen in Caenorhabditiselegans)  ...[more]

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