Proteomics

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Identification of LMAN1 and SURF4 dependent secretory cargoes


ABSTRACT: We used TMT-MS to compare protein abundance in conditioned media and cell lysates collected from control, LMAN1- or SURF4-deficient HuH7 cells to identify proteins that depend on these cargo receptors for secretion

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: David Ginsburg  

PROVIDER: MSV000092642 | MassIVE | Fri Aug 11 14:09:00 BST 2023

SECONDARY ACCESSION(S): PXD044517

REPOSITORIES: MassIVE

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Identification of LMAN1- and SURF4-Dependent Secretory Cargoes.

Tang Vi T VT   Abbineni Prabhodh S PS   Veiga Leprevost Felipe da FD   Basrur Venkatesha V   Khoriaty Rami R   Emmer Brian T BT   Nesvizhskii Alexey I AI   Ginsburg David D  

Journal of proteome research 20231016 11


Most proteins secreted into the extracellular space are first recruited from the endoplasmic reticulum into coat protein complex II (COPII)-coated vesicles or tubules that facilitate their transport to the Golgi apparatus. Although several secreted proteins have been shown to be actively recruited into COPII vesicles and tubules by the cargo receptors LMAN1 and SURF4, the full cargo repertoire of these receptors is unknown. We now report mass spectrometry analysis of conditioned media and cell l  ...[more]

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