Dataset Information

Peptide standard acquired with the dual-gated SLIM-Orbitrap

ABSTRACT: Raw data collected on the dual-gated SLIM-Orbitrap. The sample contained a mixture of standard peptides at 1 uM equimolar concentration in 60:40 water:acetonitrile with 1% acetic acid (v/v). The peptides were kemptide, angiotensin I, bradykinin, neurotensin, angiotensin II, renin substrate tetradecapeptide procine, melittin, substance P, and fibrinopeptide A. Acquisition parameters included: starting arrival time of 86 ms, 1 ms / step, width of 1 ms, 5 scans per step, MS1 only.

INSTRUMENT(S): Q-Exactive Plus with 11-meter SLIM

ORGANISM(S): Peptide Standards

SUBMITTER: Adam L. Hollerbach

PROVIDER: MSV000092738 | MassIVE | Wed Aug 23 17:08:00 BST 2023

REPOSITORIES: MassIVE

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Publications

A Dual-Gated Structures for Lossless Ion Manipulations-Ion Mobility Orbitrap Mass Spectrometry Platform for Combined Ultra-High-Resolution Molecular Analysis.

Hollerbach Adam L AL Ibrahim Yehia M YM Meras Vanessa V Norheim Randolph V RV Huntley Adam P AP Anderson Gordon A GA Metz Thomas O TO Ewing Robert G RG Smith Richard D RD

Analytical chemistry 20230612 25

High-resolution ion mobility spectrometry-mass spectrometry (HR-IMS-MS) instruments have enormously advanced the ability to characterize complex biological mixtures. Unfortunately, HR-IMS and HR-MS measurements are typically performed independently due to mismatches in analysis time scales. Here, we overcome this limitation by using a dual-gated ion injection approach to couple an 11 m path length structures for lossless ion manipulations (SLIM) module to a Q-Exactive Plus Orbitrap MS platform. ...[more]

PMID: 37307303

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