Project description:This SuperSeries is composed of the following subset Series: GSE17276: Transcriptional profiling of an evolved polymorphism in E. coli GSE17277: Array comparative genome hybridization of an evolved polymorphism in E. coli Refer to individual Series
Project description:Bulk RNA-sequencing was performed on E. coli BL21 (DE3) evolved at 25°C in pH 9 terrific broth media buffered with Tris-HCl (pH 9). The evolved E. coli was characterised and compared to the parent strain during protein expression; the strains were actively grown and compared for gene expression at pH 7 and pH 9 in terrific broth media.
Project description:We created a mutator protein. The mutator, was prepared by fusing a PmCDA1 (Petromyzon marinus Cytidine DeAminase) and E.coli RNA polymerase alpha subunit(EcoRNAP alpha). After 120 cycles, whole genome sequencing was performed on the wild type and evolved sample. After characterization of the mutation capacity of our mutator, we evolved a sucrose utilization strain and we sequenced Suc strain.
Project description:mRNA sequencing was performed on E. coli evolved at 37C on M9 Minimal Media with Glucose Growth of evolved E. coli was characterized under the same conditions as the evolution and compared to the wild type parent strain
Project description:Efficient utilization of lignocellulosic biomass-derived sugars is essential to improve the economics of biorefinery. While Pseudomonas putida is a promising microbial host, its usage is limited because this strain cannot utilize xylose or galactose as a sole carbon source. To address this issue, we heterologously introduced a xylose utilizing gene (xylD) from Caulobacter crescentus and a galactose operon (galETKM) from E. coli MG1655. To improve the utilization further, we evolved the engineered strains in minimal medium conditions. After the evolution, they acquired better fitnesses on the non-native sugars. To understand transcriptional changes after the evolution, the transcriptomes of few evolved isolates were analyzed.