ABSTRACT: This project evaluates the immune regulating properties of maple syrup extract in spleen samples utilizing an LPS induced peritonitis mouse model.
Project description:In the LPS-induced innate immune response, the expression level of IL-10 in the spleen of mice was significantly increased. The expression of this key anti-inflammatory cytokine is regulated by local sympathetic projections; however, the cell type in which it is expressed is unknown. Therefore, in this study, we used the LPS-induced mouse spleen model to analyze the spatial transcriptome, and found that IL10+ cells were mainly B cells. In addition, single-cell sequencing analysis of B cells isolated from the spleen of mice also showed that they expressed IL10. These results collectively suggest that B cells are the predominant cell type expressing IL10 in mouse spleen.
Project description:total RNA from mouse (male c57BL/6) spleen labeled with Cy3 vs total RNA from mouse (male c57BL/6) B cells treated with Lipopolysaccharide (LPS) labeled with Cy5- time course with repeats Keywords: ordered
Project description:The goat of this project is to exploreAlginate oligosaccharides (AOS) effect on the recovery of spleen function. RNA-seq of spleen samples from different groups: Con, busulfan(B), AOS, B+AOS.