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Mouse spleen LC-MSMS: effects of age and immune challenge

ABSTRACT: Ageing is characterized by the progressive lowering of the acute innate immune response and the progressive up-regulation of low-grade inflammation (i.e. inflammaging). At the cellular level, telomeres are considered as a mark of ageing as their length is progressively shortened. However, the links between telomeres and innate immune ageing remain undefined. Using a proteomic approach conducted on laboratory mice, we tested whether the experimental triggering of an acute innate response is deleterious for telomere maintenance, and whether this is dependent of age. To do so, we challenged young and old mice using bacterial lipopolysaccharide (LPS) and measured individuals’ end-points of telomere length and proteomic profiles in spleen tissue.

INSTRUMENT(S): impact HD

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Spleen

SUBMITTER: Fabrice BERTILE

LAB HEAD: Sarah Cianférani

PROVIDER: PXD009005 | Pride | 2019-03-12

REPOSITORIES: Pride

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Age-related response to an acute innate immune challenge in mice: proteomics reveals a telomere maintenance-related cost.

Criscuolo François F Sorci Gabriele G Behaim-Delarbre Margaux M Zahn Sandrine S Faivre Bruno B Bertile Fabrice F

Proceedings. Biological sciences 20181205 1892

Ageing is characterized by the impairment of the acute innate immune response and the upregulation of low-grade inflammation, i.e. inflammaging. At the cellular level, telomeres are considered as a marker of biological ageing as their length is progressively eroded in the absence of repair mechanisms. However, the link between telomeres and inflammaging remains underexplored. We aimed to identify proteins that are differentially expressed between age classes in response to an acute inflammatory ...[more]

PMID: 30518572

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