Proteomics

Dataset Information

0

O-GlcNAc Containing Proteins in Normal and Idiopathic Pulmonary Fibrosis Human Fibroblasts

ABSTRACT: Isolated normal and IPF fibroblasts were homogenized in cold MilliQ water using a bullet blender. Samples were centrifuged and inhibitors were added: HALT (Thermo Fisher Scientific), Z-Pugnac (Tocris), Thiamet G (Cayman Chemicals), and benzonase (E1014, Millipore, Sigma). O-GlcNAc enzymatic labeling and protein capturing was performed as described using a Click-IT enrichment kit following the manufacturer's protocol (cat no: C33368, C33372, and C10416; Thermo Fisher Scientific). Following an alkyne agarose bead enrichment, proteins were reduced (5 mM DTT, 30 min at 37 C), alkylated (40 mM iodoacetamide, 1 h at 37C) and digested with trypsin (1:50 enzyme/protein ratio). 0.5 ug of samples were analyzed by LC-MS/MS on a Q-Exactive HF-X mass spectrometer. Data was searched using MSFragger in match between runs mode.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jennifer E. Kyle  

PROVIDER: MSV000093453 | MassIVE | Tue Nov 21 15:52:00 GMT 2023

REPOSITORIES: MassIVE

Json Xml

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1
altmetric image

Publications

<h4>Background</h4>Idiopathic pulmonary fibrosis (IPF) is a chronic pulmonary disease that is characterized by an excessive accumulation of extracellular matrix (ECM) proteins (e.g. collagens) in the parenchyma, which ultimately leads to respiratory failure and death. While current therapies exist to slow the progression, no therapies are available to resolve fibrosis.<h4>Methods</h4>We characterized the O-linked N-Acetylglucosamine (O-GlcNAc) transferase (OGT)/O-GlcNAc axis in IPF using single-  ...[more]

Similar Datasets

Transcriptomics Urinary exosome microRNA signatures as a non-invasive prognostic biomarker for prostate cancer
2021-06-07 | GSE173094 | GEO
Transcriptomics Transcriptome analysis of primary human hepatocytes-derived organoids [RNA-seq]
2025-01-15 | GSE228249 | GEO
Genomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas (CNV)
2019-08-23 | GSE119042 | GEO
Transcriptomics Thermo Fisher Scientific OncoScan expression array data for undifferentiated uterine sarcomas (expression)
2019-08-23 | GSE119041 | GEO
Transcriptomics CIRCULATING SERUM MICRO-RNA AS NON-INVASIVE DIAGNOSTIC BIOMARKERS OF ENDOMETRIOSIS
2024-10-30 | GSE279435 | GEO
Transcriptomics Transcriptome analysis of regional idiopathic pulmonary fibrosis airway epithelial cultures
2022-07-01 | GSE205523 | GEO
Transcriptomics Elucidating the Role of the Desmosome Protein p53 Apoptosis Effector Related to PMP-22 in Growth Hormone Tumors
2022-09-29 | GSE214226 | GEO
Proteomics Hepatitis C virus induces HuR relocalization by coordinating PKC-δ and AMPK-α function
2023-09-16 | PXD035903 | Pride
Proteomics PLASMA biomarkers of Idiopathic Pulmonary Fibrosis
2020-05-13 | PXD010965 | Pride
Transcriptomics Validated Gene Expression Signatures of Idiopathic Pulmonary Fibrosis
2011-11-01 | E-GEOD-24206 | biostudies-arrayexpress