Selective Gas Phase Enrichment of Modified Peptides for Activity-Based Protein Profiling

ABSTRACT: Gas phase enrichment separates ionized peptides by their physicochemical properties, offering both simplified sample processing and integration with existing MS technology. We leveraged differential ion mobility of labeled peptides to develop a gas phase enrichment approach for reactive amino acid profiling. A compact cysteine-reactive reagent dramatically increases the ion mobility of labeled peptides and enables their selective fragmentation and sequencing.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jacob B. Geri  

PROVIDER: MSV000095231 | MassIVE | Wed Jul 03 07:05:00 BST 2024

REPOSITORIES: MassIVE