Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA from non-shock (room temperature) Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.
Project description:Many genes involve in pathogenicity and virulence are induced only in plant or in the presence of host components. Bean leaf extract was obtained from healthy bean leaves. In this work we investigated the effect of bean leaf extract on the transcriptomic profile of the bacterium, when grown at low temperature in minimal medium with or without extract from healthy bean leaves.
Project description:The plant leaves were incubated (twice) at room temperature in NaCl solution (1M, 5 mL) for 15 min each, in methanol (5 mL), then in methanol-chloroform (1:1, 5 mL) twice for 30 min, and finally washed with methanol (5 mL). After each incubation, the supernatant was discarded.
The residue was dried in the open air and submitted to alkaline hydrolysis for 18 h at room temperature and in absence of light using NaOH (1M, 50 mL g-1). The alkaline extract was filtered and acidified with HCl until pH 3 and filtered again. The extract was submitted to the same process of cleanup with SPE described above, but the column was washed with ultrapure water and methanol 5% before elution using 10 mL of methanol.
Project description:P. syringae pv. phaseolicola is the causal agent of the halo blight disease of beans (Phaseolus vulgaris L). The disease attacks both foliage and pods of plant host. Many genes involve in pathogenicity and virulence are induced only in plant or in the presence of host components. In this work we investigated the effect of bean pod extract on the transcriptomic profile of the bacterium, when grown at low temperature in minimal medium with or without bean pod extract.
Project description:Despite advancements in cancer therapy, metastasis and chemoresistance remain critical challenges due to tumor heterogeneity and compensatory pathways. This study investigates the synergistic anti-tumor potential of Flos Sophorae Immaturus (FSI) and broccoli seed extract (BSE), two natural products aligned with the "medicinal food homology" paradigm. Using in vitro and in vivo models of lung and prostate cancers, we demonstrate that FSI-BSE (F/B extract) co-treatment exerts supra-additive effects by concurrently inducing G2/M cell cycle arrest and suppressing epithelial-mesenchymal transition (EMT). Mechanistically, with integrative LC-MS/MS, network pharmacology, molecular docking, transcriptomic profiling, and molecular experimental validation, this study revealed that F/B extract targets and disrupts the cell cycle regulators such as cyclin-dependent kinases (CDKs) and cell division cycle 25 (CDC25), as well as the EMT effectors (e.g., Matrix metalloproteinase, Snail, and E-cadherin). Additionally, F/B extract suppressed tumor growth without toxicity in xenograft mouse models, supported by unaltered organ histology and blood parameters. These findings demonstrate a polypharmacological strategy leveraging dietary botanicals to address cancer complexity. By bridging cell cycle arrest and EMT inhibition, F/B extract treatment offers a non-toxic adjuvant approach with translational potential, underscoring the therapeutic value of medicinal food homology in oncology.
Project description:Along with lipidomic and metabolomic analyses, we analysed the effect of short-term heat stress on Nicotiana tabacum pollen tubes. Tubes were either grown for 3 hours at room temperature, for 6 hours at room temperature or for 3 hours at room temperature and then 37 °C for another 3 hours.
Project description:The purpose of this experiment was to identify the genes bound by Ndt80 in S. cerevisiae during meiosis and sporulation. Ndt80 was tagged with c-myc and the protein was immunoprecipitated with a c-myc antibody. Cells were grown in liquid YPA (2% Peptone, 2% Potassium Acetate, 1% Yeast Extract) at room temperature for 22 hours. Each experiment was repeated twice and sequenced on an Illumina HiSeq 4000.
Project description:We report the transcriptional changes associated with toxic effects of methanolic coal dust extract on normal zebrafish development. Early exposure of wild type embryos at 4 hpf to coal dust extract led to 3 groups of malformed phenotypes - tail deformity (P1), deformed yolk (P2) and smaller embryos with extruded yolks (P3). RNAseq of each phenotypic group revealed changes in genes involved in xenobiotic metabolism, intermediate filament composition, oxidation-reduction processes, calcium ion binding, focal adhesion and the ECM-receptor interaction pathway.