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Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster at room temperature

ABSTRACT: Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA from non-shock (room temperature) Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE22335 | GEO | 2011/01/05

SECONDARY ACCESSION(S): PRJNA127509

REPOSITORIES: GEO

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Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster during heat shock

Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster. Heat shock factor IP DNA or Mock IP DNA from heat shocked Kc 167 cells compared to whole cell extract on Agilent 2x244k tiling arrays.

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Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster

Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster using Heat Shock factor IP or Mock IP vs Whole cell Extract on Agilent 2x244k tiling arrays Heat Shock Factor IP or MOCK IP vs Whole Cell Extract

2011-01-05 | E-GEOD-19744 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Whole-genome analysis of heat shock factor binding sites in Drosophila melanogaster at room temperature

| PRJNA127509 | ENA

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Project description:ecdysone treated KC167 cells during Heat Shock and at Room Temperature

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Heat shock stimulates genomic instability and phenotypic variations in yeast

Project description:Yeast Saccharomyces cerevisiae has been widely used as a model system for studying genomic instability. In this study, heat-shock-induced genomic alterations were explored in the heterozygous diploid yeast strain JSC25-1. In combination of the whole-genome microarray, the patterns of chromosomal instability induced by heat shock could also be explored at a whole genome level. Using this system, we found heat-shock treatment resulted in hundreds-fold higher rate of genomic alterations, including aneuploidy and loss of heterozygosity (LOH).

2020-05-18 | GSE150711 | GEO

In(1)BM2(reinverted) males: reared at room temperature vs. Cold shock treatment

Project description:Cold exposure leads to alteration in the structure of the male sex chromosome of the mutant strain In(1)BM2(reinverted). Genome wide expression profiling was used to identify candidate genes involved in the expression of this phenotype. Adult male flies were exposed to cold shock at 12Â±1Â°C for 4 hours and the differentially expressed genes in the strain was compared to similarly exposed wild type Oregon R males. The microarray data was further validated using real time PCR. Two-condition experiment, RT vs. CS flies. Biological replicates: 2 control replicates, 2 replicates exposed to cold shock.

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