Transcriptomics,Genomics

Dataset Information

50

Ecdysone treated KC167 cells during Heat Shock and at Room Temperature


ABSTRACT: ecdysone treated KC167 cells during Heat Shock and at Room Temperature Overall design: 2-colour microarray desgin using 9 spotted oligonucleotide microarrays printed at the Canadian Drosophila Microarray Centre. Total RNA from Ecdysone treated KC167 cells were compared to Total RNA from an EtOH (the solvent for ecdysone) control sample pool.

INSTRUMENT(S): CDMC_Drosophila_12k1

SUBMITTER: Zak Razak  

PROVIDER: GSE23824 | GEO | 2011-08-17

SECONDARY ACCESSION(S): PRJNA130565

REPOSITORIES: GEO

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Publications

Whole-genome analysis reveals that active heat shock factor binding sites are mostly associated with non-heat shock genes in Drosophila melanogaster.

Gonsalves Sarah E SE   Moses Alan M AM   Razak Zak Z   Robert Francois F   Westwood J Timothy JT  

PloS one 20110114 1


During heat shock (HS) and other stresses, HS gene transcription in eukaryotes is up-regulated by the transcription factor heat shock factor (HSF). While the identities of the major HS genes have been known for more than 30 years, it has been suspected that HSF binds to numerous other genes and potentially regulates their transcription. In this study, we have used a chromatin immunoprecipitation and microarray (ChIP-chip) approach to identify 434 regions in the Drosophila genome that are bound b  ...[more]

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