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A Chemoselective Enrichment Strategy for In-Depth Coverage of the Methyllysine Proteome

ABSTRACT: There are the raw data of Kme1 peptides identification in the paper titled "A Chemoselective Enrichment Strategy for In-Depth Coverage of the Methyllysine Proteome".

INSTRUMENT(S): timsTOF Pro 2, Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606) Mus Musculus (ncbitaxon:10090)

SUBMITTER: Mingxuan Wu

PROVIDER: MSV000095469 | MassIVE | Sun Jul 28 20:08:00 BST 2024

REPOSITORIES: MassIVE

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A Chemoselective Enrichment Strategy for In-Depth Coverage of the Methyllysine Proteome.

Yan Lufeng L Zheng Manqian M Fan Mingzhu M Yao Rui R Zou Kun K Feng Shan S Wu Mingxuan M

Angewandte Chemie (International ed. in English) 20240910 41

Proteomics is a powerful method to comprehensively understand cellular posttranslational modifications (PTMs). Owing to low abundance, tryptic peptides with PTMs are usually enriched for enhanced coverage by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS). Affinity chromatography for phosphoproteomes by metal-oxide and pan-specific antibodies for lysine acetylome allow identification of tens of thousands of modification sites. Lysine methylation is a significant PTM; however ...[more]

PMID: 39011605

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