Acquisition of embryogenic competence during protoplast development of Fagopyrum

ABSTRACT: Reprogramming in protoplast cultures is a key step for acquiring embryogenic competence by dedifferentiated cells, thus leading to plant regeneration. Therefore, to better understand factors trigging the processes, this work focuses on analysing proteomes, cell wall composition and changes in the expression profile of selected genes. The results demonstrate a significant accumulation of somatic embryogenesis related proteins like late embryogenesis abundant proteins (embryogenic protein-DC-8-like, seed biotin-containing protein) and endochitinases during the developmental path of protoplast cultures supporting somatic embryogenesis events. Additionally, there is an increase in seed storage proteins like vicilin, oleosins, and seed biotin-containing proteins in the cultures. Investigation of somatic embryogenesis-associated transcription factors revealed intensive up-regulation of LEC1 for 50th day of F. tataricum protoplast cultures. Furthermore, we demonstrated the variable distribution of cell wall components like pectin side chains, arabinogalactan proteins (AGP) and extansin (EXT), indicating the acquisition of competence and cellular differentiation.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Fagopyrum Esculentum (ncbitaxon:3617) Fagopyrum Tataricum (ncbitaxon:62330)

SUBMITTER: Artur Pinski   Alexander Betekhtin  

PROVIDER: MSV000095861 | MassIVE |

SECONDARY ACCESSION(S): PXD055850

REPOSITORIES: MassIVE