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Development of subunit-selective proteasome substrates for Schistosoma species by MSP-MS

ABSTRACT: Development of optimized subunit-specific substrates for S. mansoni proteasome (Sm20S) based on data generated by Multiplex Substrate Profiling by Mass Spectrometry (MSP-MS). MSP-MS was performed with a library of 228 synthetic tetradecapeptides. Sm20S was incubated for 1h and 3h with Bortezomib, Carfilzomib, DMSO (vehicle control), and the absence of inhibitor or vehicle control (time 0 samples). Assays were conducted in quadruplicate by incubating Sm20S with the peptide library.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Schistosoma Mansoni (ncbitaxon:6183)

SUBMITTER: Anthony ODonoghue

PROVIDER: MSV000096255 | MassIVE | Wed Oct 30 13:52:00 GMT 2024

SECONDARY ACCESSION(S): PXD057369

REPOSITORIES: MassIVE

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