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Quorum sensing modulates microbial community structure through regulation of secondary metabolites

ABSTRACT: Bacteria are recognized for their diverse metabolic capabilities, yet the impact of microbe-microbe interactions on multispecies community structure and dynamics is poorly understood. Cell-to-cell signaling in the form of quorum sensing (QS) often regulates secondary metabolite production and microbial interactions. Here we examine how acylhomoserine lactone (AHL) mediated QS impacts microbial community structure in a 10-member synthetic community of isolates from Populus deltoides. To explore the role of QS in microbial community structure and dynamics, we disrupted AHL signaling using purified AiiA-lactonase, an enzyme that cleaves the lactone ring. Microbial community structure resulting from signal inactivation, as measured by 16S amplicon sequencing and secondary metabolite production, was assessed after successive passaging of the community. Further, we investigated the impact of quorum quenching on microbe-microbe interactions using pairwise inhibition assays. Our results indicate that AHL inactivation alters the relative abundance of dominant community members at later passages but does not impact the overall membership in the community. Quorum quenching significantly alters the metabolic profile in AiiA-lactonase treated communities. This metabolic alteration impacts microbe-microbe interactions through decreased inhibition of other community members. Together, these results indicate that QS impacts microbial community structure through the regulation of secondary metabolites in dominant members and that membership of microbial communities can be relatively stable despite changes in metabolic profiles

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Pseudomonas (ncbitaxon:286) Paraburkholderia Sp. (ncbitaxon:1926495) Bacillus <bacterium> (ncbitaxon:1386) Variovorax (ncbitaxon:34072) Duganella (ncbitaxon:75654) Caulobacter (ncbitaxon:75) Sphingobium (ncbitaxon:165695) Rhizobium Streptomyces (ncbitaxon:1883) Pantoea (ncbitaxon:53335)

SUBMITTER: Paul Abraham

PROVIDER: MSV000096353 | MassIVE | Fri Nov 08 09:03:00 GMT 2024

REPOSITORIES: MassIVE

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Quorum sensing modulates microbial community structure through regulation of secondary metabolites

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