Quantitative proteomic analysis reveals JMJD6 and DNAJB11 as endogenous substrates of E3 ligase RFFL

ABSTRACT: Immunoprecipitation experiments using anti-RFFL antibody. Samples were loaded in technical duplicates in EvoTips, and chromatographic separation was done on an in-house column and the 30 samples per day (SPD) method on Evosep One system (Evosep). Peptides were detected and fragmented using timsTOF Pro 2 mass spectrometer (Bruker Daltonics) in data-dependent mode with parallel accumulation serial fragmentation (PASEF) enabled. The raw data was analyzed using Fragpipe (v 22.0). MS/MS spectra were searched using MSFragger (v 4.1) against forward and reversed Homo sapiens UniProt sequence database (UP000005640) supplemented with common contaminants (total 20,979 sequences excluding decoys).

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Prof. S. Murty Srinivasula  

PROVIDER: MSV000096868 | MassIVE | Thu Jan 16 02:32:00 GMT 2025

SECONDARY ACCESSION(S): PXD059871

REPOSITORIES: MassIVE