An intrinsically disordered region of histone demethylase KDM5A regulates catalysis through interactions with the nucleosomal acidic patch and DNA

ABSTRACT: KDM5A lysine demethylase conformation was probed by BS3 crosslinking in the presence and absence of DNA. Crosslinked proteins were reduced, alkylated with iodoacetamide, trypsin digest, and labeled with TMT6plex reagent prior to mixing and acquisition on an Orbitrap Exploris equiped with a FAIMS source. Three biological replicates are included split over multipe technical replicates and/or SEC fractions. The SampleKey.txt file explains the labeling scheme.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Danica Galonic Fujimori  

PROVIDER: MSV000097094 | MassIVE | Mon Feb 10 14:49:00 GMT 2025

SECONDARY ACCESSION(S): PXD055029

REPOSITORIES: MassIVE