Proteomics of bacterial and ovarian cancer ascites extracellular vesicles

ABSTRACT: Extracellular vesicles (EVs) are present in body fluids, and they can be disease biomarkers. Emerging evidence has proven that EVs are released not only from mammalian cells but also from bacteria. Ovarian cancer has a dismal prognosis because of difficulties in early detection. This study aimed to identify bacterial EV (BEV) proteins associated with ovarian cancer. Fifteen patients with ovarian cancer or benign tumors were recruited, and EVs were isolated from the ascites. BEVs were recovered from seven in vitro-cultured strains of bacteria present in the vaginal microbiota, and LC-MS/MS analysis was performed. The detected peptide data were annotated to both human and bacterial references, and the human data showed that the profiles of cancer EVs were distinct from those of patients with benign tumors.

INSTRUMENT(S): LC-MS/MS

ORGANISM(S): Lactobacillus Gasseri (ncbitaxon:1596)

SUBMITTER: Akira Yokoi  

PROVIDER: MSV000097198 | MassIVE | Sun Feb 23 04:41:00 GMT 2025

SECONDARY ACCESSION(S): PXD061094

REPOSITORIES: MassIVE