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GC-MS Profile for Cultured Anaerobic Gut Fungi - Anaeromyces robustus and Caecomyces churrovis

ABSTRACT: Untargeted metabolomic profiling with gas chromatography-mass spectrometry (GC-MS) was performed for two gut fungal strains, Anaeromyces robustus S4 and Caecomyces churrovis A, enabling putative identification of metabolites and providing insights into gut fungal metabolic capabilities. Metabolites were extracted using the Metabolite, Protein, and Lipid Extraction (MPLEx) method and derivatized using a modified version of the protocol used to create FiehnLib. Samples underwent methoximation to protect carbonyl groups and reduce tautomeric isomers, followed by silylation with N-methyl-N-trimethylsilyltrifluoroacetamide and 1% trimethylchlorosilane (MSTFA) to derivatize hydroxy and amine groups to trimethylsilated (TMS) forms.

INSTRUMENT(S): Agilent GC 7890A-MSD 5975C

ORGANISM(S): Caecomyces Churrovis (ncbitaxon:2019372) Anaeromyces Robustus (ncbitaxon:1754192)

SUBMITTER: Michelle O'Malley

PROVIDER: MSV000097279 | MassIVE | Mon Mar 10 12:59:00 GMT 2025

REPOSITORIES: MassIVE

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GC-MS Profile for Cultured Anaerobic Gut Fungi - Anaeromyces robustus and Caecomyces churrovis

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